General Cell Based Assays
Cayman Chemical Phagocytosis Assay Kit (IgG-DyLight™ 633), 1 ea
Cayman’s Phagocytosis Assay Kit (IgG-DyLight™ 633) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-DyLight™ 633 Complex for up to 500 samples.
Eurofins Discoverx PathHunter eXpress CHRM4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0349E2CP0L)
The PathHunter CHRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM4 and EA-tagged β-Arrestin-2. Activation of CHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL17RA/IL17RC Dimerization Assay, 10pl Kit. (93-0999E3CP0L)
The PathHunter IL17RA/IL17RC (U2OS) Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-17a) induced dimerization of IL17RA/IL17RC (U2OS) receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 19 Reagent, 100. (93-0563R19A)
AssayComplete Cell Plating 19 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress GPR37 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0339E2ACP1L)
The PathHunter GPR37 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR37 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR37 and EA-tagged β-Arrestin-2. Activation of GPR37 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human MCP-2 ASY 25 PL
The Meso Scale Discovery U-PLEX Human MCP-2 Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of the human chemokine MCP-2 It utilizes MSD s proprietary electrochemiluminescent detection technology and is provided in a convenient all-in-one kit containing plates coated with capture antibodies detection antibodies calibrators and buffers The assay has high sensitivity and wide dynamic range requires low sample volume and provides rapid results It is intended for research use only to monitor MCP-2 levels in cell culture supernatants serum plasma and other biological samples
Abcam Glutathione Peroxidase Activity Assay Kit (Fluorometric)
Glutathione Peroxidase Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical LDH Cytotoxicity Assay Kit, 96 Well
For measurement of LDH activity as an indicator of cell death
ACROBiosystems Human Fc gamma RIIIA / CD16a (V176) binding Kit (TR-FRET) is expressed from Human, 100 tests
Human Fc gamma RIIIA / CD16a (V176) binding Kit (TR-FRET) is expressed from Human
Cayman Chemical LipidLaunch™ LNP-0315 Uptake Kit (Near-Infrared Fluorescence), 1 ea
Cayman’s LipidLaunch™ LNP-0315 Uptake Kit (Near-Infrared Fluorescence) is intended to serve as a starting point for laboratories to explore the uptake of LNPs in vivo without the need for specialized equipment. Optimal preparation conditions for the encapsulation of nucleic acids with LNPs must be determined by the end user.
Sigma Aldrich Fine Chemicals Biosciences Ras Assay Reagent Raf 1 RB
Product Source Human Ras Binding Domain (RBD residues 1-149) of Raf-1 expressed in E. coli.
Eurofins Discoverx cAMP Hunter eXpress GLP1R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0062E2CP2M)
The cAMP Hunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Indigo Biosciences INDIGO BIOSCIENCES Human FGFR4 Reporter Assay System, 1 x 384-well format, FGFR4/α-Klotho
This reporter assay system uses proprietary human cells expressing Human Fibroblast Growth Factor Receptor 4 and α-Klotho (FGFR4/α-Klotho) to quantify luciferase activity for screening functional interactions.
- Utilizes proprietary human cells engineered for constitutive expression of human fibroblast growth factor receptor 4
- Exploits signal transduction via the Ca+2·calcineurin / NFAT cascade
- Quantifies relative changes in luciferase activity for dose-dependent measure
- All-inclusive cell-based reporter assay system with cryopreserved cells for immediate dispensing
Dojindo Molecular Technologies Inc DNA DAMAGE QUANTIFIC KIT (20 SAMPLES) is a colorimetric microplate assay and can determine the number of abasic sites in genomic DNA samples.
Oxidative damage to DNA is a result of its interaction with reactive oxygen species (ROS), in particular the hydroxy radical. Hydroxy radicals, which are produced from superoxide anion and hydrogen peroxide by the Fenton reaction, produce multiple modifications in DNA. Oxidative attacks by hydroxy radicals on the deoxyribose moiety leads to the release of free bases from DNA, generating strand breaks with various sugar modifications and simple abasic sites (AP sites). In fact, AP sites are one of the major types of damage generated by ROS. The Aldehyde Reactive Probe reacts specifically with an aldehyde group present on the open ring form of the AP sites. DNA Damage Quantification Kit contains all the necessary solutions for detecting between 1 to 40 AP sites per 1 x 105 base pairs.